Biochemistry I
Outline
DNA double-strand break (DSB) is one of the most serious threats for cells. Improper repair of DSBs leads to cell death or carcinogenesis. DSBs can be induced by exogenous stimuli such as ionizing radiation, and endogenous normal cellular processes such as DNA replication, meiosis and V(D)J recombination. Since we found 53BP1 as a tumor suppressor p53-binding protein in 1994, many laboratories, including ours, have shown that 53BP1 is involved in cellular responses to DSBs.
Goals in our research are:
1) clarifying molecular mechanisms for the cell-cycle checkpoints and repair of DSBs.
2) generating a novel method in radiation therapy for cancer stem cells.
e-mail: biochem1@kanazawa-med.ac.jp
Faculty
Professor and Chair
Senior Assistant Professor
Senior Assistant Professor
Assistant Professor
Secretary
TANIHARA Kiyomi
Research Achievements
Shibata T, Ikawa M, Sakasai R, Ishigaki Y, Kiyokawa E, Iwabuchi K, Singh DP, Sasaki H, Eri Kubo E. Lens–specific conditional knockout of Tropomyosin 1 gene in mice causes abnormal fiber differentiation and lens opacity. Mech Ageing Dev. 2021, 196: 111492.
Fujii A, Sunatani Y (Co-1st author), Furuichi K, Fujimoto K, Adachi H, Iwabuchi K, Yokoyama H. DNA damage in human glomerular endothelial cells induces nodular glomerulosclerosis via an ATR and ANXA2 pathway. Sci Rep. 2020, 10(1): 22206.
Matsui M, Sakasai R, Abe M, Kimura Y, Kajita S, Torii W, Katsuki Y, Ishiai M, Iwabuchi K, Takata M, Nishi R. USP42 enhances homologous recombination repair by promoting Rloop resolution with a DNA–RNA helicase DHX9. Oncogenesis. 2020, 9(6): 60.
Sunatani Y, Kamder RP, Sharma MK, Matsui T, Sakasai R, Hashimoto M, Ishigaki Y, Matsumoto Y, Iwabuchi K. Caspase-mediated cleavage of X-ray repair cross-complementing group 4 promotes apoptosis by enhancing nuclear translocation of caspase-activated DNase. Exp. Cell Res. 2018, 362(2): 450-460.
Sakasai R, Isono M, Wakasugi M, Hashimoto M, Sunatani Y, Matsui T, Shibata A, Matsunaga T, Iwabuchi K. Aquarius is required for proper CtIP expression and homologous recombination repair. Sci. Rep. 2017, 7(1): 13808.
External Research Funding
Grants-in-aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (21H03600). 2021-2023, Iwabuchi K.
Grants-in-aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (21K12245). 2021-2023, Sakasai R.
Grants-in-aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (20K12170). 2020-2022, Matsui T.
Grant-in-Aid for Research Activity Start-up (20K23366). 2020-2021, Sunatani Y.
Grants-in-aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (18H03375). 2018-2020, Iwabuchi K.